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Anti Ki67, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Ki 67 Cell Signaling, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ki67  (Cell Signaling Technology Inc)
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( A ) CD45 − CD24 + CD29 hi MECs were isolated from the mammary glands of 12-week-old Ptpn2 fl/fl and MMTV-Cre; Ptpn2 fl/fl females and transplanted contralaterally into the cleared mammary fat pads of 3-week-old FVB/n female mice. The transplanted fat pads were collected after 12 weeks and fixed in formalin, and ( B ) whole mounts stained with carmine alum to assess epithelial repopulation or processed for either ( C ) immunohistochemistry and stained for PTPN2, STAT-3 Y705 phosphorylation (p-STAT-3), and <t>Ki67</t> (counterstained with hematoxylin) or ( D and E ) immunofluorescence microscopy and stained for CK8, CK14, and DNA [4′,6-diamidino-2-phenylindole (DAPI)] to identify nuclei. In (C), p-STAT-3– or <t>Ki67-positive</t> MECs were quantified; three mice per genotype were scored, with three randomly selected fields of view scored per section. (E) Quantification of perturbed ductal structures in Ptpn2 fl/fl and MMTV-Cre; Ptpn2 fl/fl MEC outgrowths; four repopulated fat pads per mouse were scored per genotype with at least five randomly selected fields of view and 50 structures scored per gland. Results in (C) and (D) are means ± SEM; significance was determined using two-tailed Student’s t tests; * P ≤ 0.05; *** P ≤ 0.001.
Rabbit Ki67, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit ki67/product/Cell Signaling Technology Inc
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( A ) CD45 − CD24 + CD29 hi MECs were isolated from the mammary glands of 12-week-old Ptpn2 fl/fl and MMTV-Cre; Ptpn2 fl/fl females and transplanted contralaterally into the cleared mammary fat pads of 3-week-old FVB/n female mice. The transplanted fat pads were collected after 12 weeks and fixed in formalin, and ( B ) whole mounts stained with carmine alum to assess epithelial repopulation or processed for either ( C ) immunohistochemistry and stained for PTPN2, STAT-3 Y705 phosphorylation (p-STAT-3), and <t>Ki67</t> (counterstained with hematoxylin) or ( D and E ) immunofluorescence microscopy and stained for CK8, CK14, and DNA [4′,6-diamidino-2-phenylindole (DAPI)] to identify nuclei. In (C), p-STAT-3– or <t>Ki67-positive</t> MECs were quantified; three mice per genotype were scored, with three randomly selected fields of view scored per section. (E) Quantification of perturbed ductal structures in Ptpn2 fl/fl and MMTV-Cre; Ptpn2 fl/fl MEC outgrowths; four repopulated fat pads per mouse were scored per genotype with at least five randomly selected fields of view and 50 structures scored per gland. Results in (C) and (D) are means ± SEM; significance was determined using two-tailed Student’s t tests; * P ≤ 0.05; *** P ≤ 0.001.
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( A ) CD45 − CD24 + CD29 hi MECs were isolated from the mammary glands of 12-week-old Ptpn2 fl/fl and MMTV-Cre; Ptpn2 fl/fl females and transplanted contralaterally into the cleared mammary fat pads of 3-week-old FVB/n female mice. The transplanted fat pads were collected after 12 weeks and fixed in formalin, and ( B ) whole mounts stained with carmine alum to assess epithelial repopulation or processed for either ( C ) immunohistochemistry and stained for PTPN2, STAT-3 Y705 phosphorylation (p-STAT-3), and <t>Ki67</t> (counterstained with hematoxylin) or ( D and E ) immunofluorescence microscopy and stained for CK8, CK14, and DNA [4′,6-diamidino-2-phenylindole (DAPI)] to identify nuclei. In (C), p-STAT-3– or <t>Ki67-positive</t> MECs were quantified; three mice per genotype were scored, with three randomly selected fields of view scored per section. (E) Quantification of perturbed ductal structures in Ptpn2 fl/fl and MMTV-Cre; Ptpn2 fl/fl MEC outgrowths; four repopulated fat pads per mouse were scored per genotype with at least five randomly selected fields of view and 50 structures scored per gland. Results in (C) and (D) are means ± SEM; significance was determined using two-tailed Student’s t tests; * P ≤ 0.05; *** P ≤ 0.001.
Granzyme B 46890, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ki 67  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc ki 67
( A ) CD45 − CD24 + CD29 hi MECs were isolated from the mammary glands of 12-week-old Ptpn2 fl/fl and MMTV-Cre; Ptpn2 fl/fl females and transplanted contralaterally into the cleared mammary fat pads of 3-week-old FVB/n female mice. The transplanted fat pads were collected after 12 weeks and fixed in formalin, and ( B ) whole mounts stained with carmine alum to assess epithelial repopulation or processed for either ( C ) immunohistochemistry and stained for PTPN2, STAT-3 Y705 phosphorylation (p-STAT-3), and <t>Ki67</t> (counterstained with hematoxylin) or ( D and E ) immunofluorescence microscopy and stained for CK8, CK14, and DNA [4′,6-diamidino-2-phenylindole (DAPI)] to identify nuclei. In (C), p-STAT-3– or <t>Ki67-positive</t> MECs were quantified; three mice per genotype were scored, with three randomly selected fields of view scored per section. (E) Quantification of perturbed ductal structures in Ptpn2 fl/fl and MMTV-Cre; Ptpn2 fl/fl MEC outgrowths; four repopulated fat pads per mouse were scored per genotype with at least five randomly selected fields of view and 50 structures scored per gland. Results in (C) and (D) are means ± SEM; significance was determined using two-tailed Student’s t tests; * P ≤ 0.05; *** P ≤ 0.001.
Ki 67, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A ) CD45 − CD24 + CD29 hi MECs were isolated from the mammary glands of 12-week-old Ptpn2 fl/fl and MMTV-Cre; Ptpn2 fl/fl females and transplanted contralaterally into the cleared mammary fat pads of 3-week-old FVB/n female mice. The transplanted fat pads were collected after 12 weeks and fixed in formalin, and ( B ) whole mounts stained with carmine alum to assess epithelial repopulation or processed for either ( C ) immunohistochemistry and stained for PTPN2, STAT-3 Y705 phosphorylation (p-STAT-3), and Ki67 (counterstained with hematoxylin) or ( D and E ) immunofluorescence microscopy and stained for CK8, CK14, and DNA [4′,6-diamidino-2-phenylindole (DAPI)] to identify nuclei. In (C), p-STAT-3– or Ki67-positive MECs were quantified; three mice per genotype were scored, with three randomly selected fields of view scored per section. (E) Quantification of perturbed ductal structures in Ptpn2 fl/fl and MMTV-Cre; Ptpn2 fl/fl MEC outgrowths; four repopulated fat pads per mouse were scored per genotype with at least five randomly selected fields of view and 50 structures scored per gland. Results in (C) and (D) are means ± SEM; significance was determined using two-tailed Student’s t tests; * P ≤ 0.05; *** P ≤ 0.001.

Journal: Science Advances

Article Title: PTPN2 elicits cell autonomous and non–cell autonomous effects on antitumor immunity in triple-negative breast cancer

doi: 10.1126/sciadv.abk3338

Figure Lengend Snippet: ( A ) CD45 − CD24 + CD29 hi MECs were isolated from the mammary glands of 12-week-old Ptpn2 fl/fl and MMTV-Cre; Ptpn2 fl/fl females and transplanted contralaterally into the cleared mammary fat pads of 3-week-old FVB/n female mice. The transplanted fat pads were collected after 12 weeks and fixed in formalin, and ( B ) whole mounts stained with carmine alum to assess epithelial repopulation or processed for either ( C ) immunohistochemistry and stained for PTPN2, STAT-3 Y705 phosphorylation (p-STAT-3), and Ki67 (counterstained with hematoxylin) or ( D and E ) immunofluorescence microscopy and stained for CK8, CK14, and DNA [4′,6-diamidino-2-phenylindole (DAPI)] to identify nuclei. In (C), p-STAT-3– or Ki67-positive MECs were quantified; three mice per genotype were scored, with three randomly selected fields of view scored per section. (E) Quantification of perturbed ductal structures in Ptpn2 fl/fl and MMTV-Cre; Ptpn2 fl/fl MEC outgrowths; four repopulated fat pads per mouse were scored per genotype with at least five randomly selected fields of view and 50 structures scored per gland. Results in (C) and (D) are means ± SEM; significance was determined using two-tailed Student’s t tests; * P ≤ 0.05; *** P ≤ 0.001.

Article Snippet: Rabbit p-STAT-1 (Tyr 701 ) (clone 58D6) [Research Resource Identifier (RRID): AB_2198287), rabbit p-STAT-3 (Tyr 705 ) (clone D3A7) (RRID: AB_2255568], mouse STAT-3 (clone 124H6) (RRID: AB_331757), rabbit STAT-1 (#9172) (RRID: AB_2198300), rabbit Ki67 (clone D3B5) (RRID: AB-2687446), mouse Ki67 (clone #8D5) (RRID:AB_2797703), and rabbit Vinculin (clone E1E9V) (RRID: AB_2728768) antibodies were from Cell Signaling Technology (Danvers, MA); mouse actin (clone ACTN05) (RRID: AB_1954910) antibody was from Thermo Fisher Scientific (Waltham, MA); rabbit CK8 (clone EP1628Y) (RRID: AB_869901), mouse CK14 (clone LL002) (RRID: AB_306091), and rabbit CD3ε (clone SP7) antibodies were from Abcam (Cambridge, UK) (RRID: AB_446487); rabbit FLEX CD3ε Ready-to-Use was from Agilent Dako (RRID: AB_2732001) (Glostrup, Denmark); and mouse PTPN2 6F3 antibody was from MediMabs (Quebec, Canada).

Techniques: Isolation, Staining, Immunohistochemistry, Phospho-proteomics, Immunofluorescence, Microscopy, Two Tailed Test